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POD reacting with hydrogen peroxide (H2O2) catalyzes oxidative condensation of tyramide to provide very reactive intermediates, tyramide radicals. The activated tyramide covalently binds to electron-wealthy moieties including tyrosine, phenylalanine and tryptophan at or near the POD. The short 50 %-life of the tyramide radicals restricts the deposi

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